The purpose of this experiment was to measure the nuclear volume of exfoliated normal vaginal epithelial cells using cytologic vaginal smears stained by Papanicolaou´s method.
The specimens were obtained from 46 patients who visited Outpatient Department, Ob. & Gyn., SNUH and were free from infectious disease or neo-plastic disease of reproductive organs.
At first, the longest and shortest diameter of each cell were measured with the, "Zeiss Winkel´s Zelchen Apparatur" attached to microscope, then their nuclear volumes were calculated by Matthias, Lange & Herb´s formula.
The exfoliated vaginal epithelial cells were classified as cornified, intermediate and parabasal cells. Only the cells which stained clearly and had no vacuolation, stippling, cytolysis or karyolysis were selected for the measurement.
The results obtained are as follows:
1) The nuclear volume of the cornified cells were ranging from 13..8?^3 to 107..1^3; the intermediate cells, 66.0?^3;-528.0^3; and the parabasal cells, 110.03-1,494.03.
1) The ratio of the nuclear volume of each epithelial layer; Cornified: Intermediate: Parabasal =1:4:8
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